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- Reptiles have horny or scaly skin. Mammals have fur or hair.
- Mammals have a single type of skin cell for colour. Reptiles have 3 types of skin cell for colour.
- Reptiles have low metabolism and require less energy. Mammals have high metabolism.
- The body temperature of reptiles varies according to their environment (they are 'cold-blooded'). Mammals are able to maintain a constant temperature (they are 'warm-blooded').
- Reptiles (but not tortoise/turtles, snakes or crocodilians) have a "third eye", known as a parietal or pineal eye. It has a cornea, lens and photoreceptors. Mammals have only a parietal gland, used for endocrine production.
- Reptiles have small, relatively even teeth with single roots; they are replaced often. Mammals have different types of teeth with multiple roots - incisors, canines, premolars and molars - and replace their teeth only once.
- Reptiles have 3 bones in the lower jaw: the dentary (holding the teeth), the quadrate and the articular. Mammals have only one lower jaw bone.
- Reptiles' ears have only one bone, the stapes. Mammals have 3 bones, (stapes, malleus, incus), and the very complicated organ of Corti.
- The reproductive system of male reptiles includes a hemi- penis. This consists of two penises, which are used singly and repeatedly to fertilise several of a female's eggs.
- Most reptiles lay eggs. Mammals do not.
- Reptiles do not usually guard their eggs or care for their young. Mammals all have milk glands and suckle their young.
- The sex of the unborn young of reptiles is determined by external temperature. Hot temperatures result in more females.
- Mammals breathe by way of a diaphragm in their chest (the thorax). Reptiles have no diaphragm and breathe very differently, with their cheeks and mouth.
- Reptiles have a 3-chambered heart, except crocodilians which, like mammals, have a 4-chambered heart. Since their hearts cannot pump blood far upwards, reptiles move close to the ground (they 'creep').
- The legs of reptile are splayed out from the body. The legs of mammals are positioned under the body.
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